Insulin is a type of hormone that is excreted in the human pancreas by the islets of langerhans. Insulin is one of the important herbs in humans that is secreted from the pancreas beta cell. And reduces the high levels of glucose present in the blood to normal levels. If the pancreas does not drain or insulate the pancreas due to insufficiency or if insulin is depleted, then the blood glucose levels rise, ie diabetes mellitus. In this case the diabetic reggae has to take insulin injection. There are millions of such rabies in Bangladesh, so the demand for insulin is also huge. How to produce Insulin production ?
Basic Concepts of Insulin What is insulin?
Insulin is a small elemental protein made up of 51 amino acids. Two poly peptide chains (chains consisting of 21 amino acids – A and chains consisting of 30 amino acids – B) form an insulin molecule by connecting through two sulfide bonds. Its chemical signal is: C254H377O75S6 Molecular mass 5734.
Currently the human insulin producing gene E. Massive insulin is being produced by transferring to coli. Insulin is made up of about one million molecules in a bacterium cell. Primary treatment for fractures and fractures
Human insulin production through gene engineering
Diabetes rag treatment has a lot of insulin supply, but where is so much insulin in nature? Once insulin was collected from a cow or a pig pancreas, it was used in human treatment. But insulin taken from cows or pigs is not so useful to humans. Therefore, by taking advantage of gene engineering knowledge, the human gene is used to initiate the production of insulin by artificial means and at one time it is successful. Insulin-producing genes are first identified in human DNA. Shake it to the top of the small chromosome DNA of chromosome No. 3. It contains 3 nitrogen – the genetic code of the base insulin gene.
Eli Lily & Corparay of America invented human insulin production strategies through gene engineering and biotechnology, which was first marketed in 2002 under the name “Humulin”.
The process of insulin production
GMO E in fermentation tank. Enhancement of coli numbers: Isolation of insulin gene parts from DNA sources; Human insulin production through gene engineering.
Identification of insulin-producing genes; The insulin-producing membrane in the human body is currently located in the DNA of the upper arm of the small chromosome No. 1.
Isolation of insulin gene parts from DNA sources;
The replication enzyme is used to separate the cutaneous gene from human DNA in a special way.
Carrier Psmid Separation:
E coli specialize in the bacterium to carry the insulin gene. The prism is separated.
E. Collimation of coli plasmid DNA:
Restriction enzyme propagates like the insulin gene. The DNA portion of the passimid DNA is cut off and left in space.
Placing insulin genes in plasmid DNA:
The human insulin gene DNA fragment is inserted into the truncated space of the pramid DNA, and the pramid DNA and human DNA are attached using the ligase enzyme. The recombinant DNA or recombinant pyramid was created
Recombinant plasmid is an E. coli Entering the bacterium:
This is done in the transformation process. This is done in the heat shock method or, the electric pass method. The bacteria that carry the recombinant plasmid are called vectors. The carrier must be free of its own plasmid. The vector has to be Competent to receive the plasmid. The vector according to the hit shock method (E _ cali). CaCl first; Immersed in the solution and kept in ice for 3-5 hours. E in it. The cell wall of the coli E from the Ca leg. coli competent the cells to receive plasmids. Then E. coli mix the cells and the recombinant plasmid together in half of a container. Put on ice for 1 hour, then heat at 12 ° C for 5 seconds and again for 2 minutes. plasmid by coating the coti cells. Body. Now E. GMO E. coli cells with insulin genes. turn on coli.
GMO E in fermentation tank. coli number enhancement:
Now GMO E. coli cum transgenic e. The coti is kept in a fixed culture media containing fermentation tank at a specified temperature. Millions of transgenic E in short period of time in fermentation tank. coli is produced and the insulin produced in each cell is deposited.
Insulin is formed and stays inside the cell. Therefore, E, coti cells are lysed (dissolved) and receive insulin
Insulin, which is found by thawing the bacteria, is normal for the bacteria to have their own protein. Therefore, the extracted insulin is purified.
Insulin is marketed at the appropriate ampulle volume during production, and the injection pushes the muscle in the right amount and at the right time. Insulin in the body flows with the blood to form a suitable receptor site in the renal membrane, which allows the glucose to enter the cell and normalizes the blood glucose levels. More on Wikipedia